Traditional polymerase chain reaction (PCR) has been a vital technique for many labs since its development in the 1980s. Its advanced counterpart, real-time PCR, has also found its way into many genomics labs. As its name implies, real-time PCR, also referred to as qPCR, measures amplification as it occurs, providing even greater levels of sensitivity and accuracy. Used in numerous applications, including genotyping, copy number variation, and NGS library prep, the qPCR instrument market is expected to grow an average of 11% per year by 20181.
To accommodate the growing number of labs using qPCR and to provide an easy way for these labs to integrate real-time PCR, GenoLogics has launched integrations with LifeTechnologies/Thermo Fisher QuantStudio qPCR instruments. BaseSpace Clarity LIMS integrates with the following QuantStudio instruments:
In general, the integrations automate the placement of samples for TaqMan array plates and array cards and generate sample files for use with the QuantStudio Flex controller software. With the integration, users get:
In addition, users of the QuantStudio 12 Flex can track Open Array plate layout files and generate sample information files for use with SampleTracker software for OpenArray experiments.
Each of the three integrations comes with multiple, preconfigured workflows, summarized below.
|Workflows||QuantStudio 6 Flex||QuantStudio 7 Flex||QuantStudio 12K Flex|
|DNA Gene Expression Workflow||✔||✔||✔|
|cDNA Gene Expression Workflow||✔||✔||✔|
|DNA Genotyping Workflow||✔||✔||✔|
|cDNA Genotyping Workflow||✔||✔||✔|
|Library Prep Quantification Workflow||✔||✔||✔|
|DNA OpenArray Gene Expression Workflow||✔|
|cDNA OpenArray Gene Expression Workflow||✔|
|DNA OpenArray Genotyping Workflow||✔|
|cDNA OpenArray Genotyping Workflow||✔|
|Automatic Sample Placement for TaqMan Array Plates and Cards||✔||✔||✔|
|Sample Information File Generation for use with SampleTracker Software||✔|
|SampleTracker Plate Layout Files upload to BaseSpace Clarity LIMS for tracking purposes||✔|
|AccuFill File upload to BaseSpace Clarity LIMS for tracking purposes||✔|
|Sample Information File Generation for use with QuantStudio||✔||✔||✔|
|Raw data file upload to BaseSpace Clarity LIMS||✔||✔||✔|
The integrations are available now for all BaseSpace Clarity LIMS users. Contact GenoLogics for more information.